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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore may contribute to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to guarantee the absolute best final results are...
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This guidebook outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation designs which contribute to the temporal coordination of each mitotic celebration. Cyclin E kinds a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates for the G1-S period boundary and is also degraded as cells progress by S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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